The venue for this event will be
The Stevenage Bioscience Catalyst, within the GSK complex, Stevenage, UK
The Stevenage Bioscience Catalyst campus is a unique bioscience community created to provide small biotech and life sciences companies and start-ups with access to the expertise, networks and scientific facilities traditionally associated with multinational pharmaceutical companies.
This is a Euroscicon Small Conference, an outline of the day can be found at
Major sponsor- Applikon Biotechnology UK
Who should attend: Robotics experts, High throughput technology experts, Scale down scientists, Microscale technology scientists, Clone selection companies, Microtitre plate companies
Following on from the success of our 2010 and 2011 events, Professor Gary Lye is Chairing the third meeting in the series "Miniaturisation -Micro Scale Bioprocess Development". Miniaturisation and automation of bioprocess development continues to be a rapidly expanding area of interest since the technologies promise to reduce biopharmaceutical development time and cost. This meeting aims to highlight recent technologies used in high throughput bioprocess development, from clone selection through to analysis of final product and formulation. A series of expert speakers will describe the development and use of current miniaturisation technologies together with the technical and regulatory hurdles that must be overcome to facilitate wider industrial uptake.
Meeting Chair: Professor Gary J Lye, PhD, CEng, CSci, FIChemE, Director Industrial Doctoral Training Centre, Deputy Head of Department, The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, UK
This event has CPD accredditation
9:00 – 9:45 Registration
9:45 – 9:50 Introduction to event: Dr Glenn Robinson, Applikon Biotechnology UK
9:50 – 10:00 Introduction by the chair: Professor Gary J Lye, PhD, CEng, CSci, FIChemE, Director Industrial Doctoral Training Centre, Deputy Head of Department, The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, UK
10:00 – 10:30 Shaken or stirred – Miniature bioreactors for rapid process development
Dr Frank Baganz, Senior Lecturer, Biochemical Engineering, University College London
The need for greater speed and efficiency during the development of bioprocesses has driven the advancement of miniature bioreactor (MBR) technologies in recent years. This presentation will focus on shaken microwell-based systems and miniaturised stirred tank reactors and will cover its engineering characterisation in terms of power input, liquid phase mixing and oxygen mass transfer. Furthermore, the requirements of MBR technologies for fermentation and cell culture processes will be discussed and examples for application of MBRs in both areas given.
10:30 – 11:00 Miniaturised bioreactors- the future for small scale processes
Dr Timo Keijzer, Applikon Biotechnology, UK
11:00 – 11:30 Speakers’ photo then mid-morning break and trade show
Please try to visit all the exhibition stands during your day at this event. Not only do our sponsors enable Euroscicon to keep the registration fees competitive, but they are also here specifically to talk to you
11:30 – 11:45 Streamlining cell line evaluation using micro bioreactor systems
Dr Steve Warr, GlaxoSmithKline, Stevenage, UK
The development of processes for the production of biopharmaceutical molecules conventionally involves the generation of multiple cell lines in multiwell plates, cell line screening in shake flasks followed by final cell line selection and process optimisation in bioreactors. Despite the use of platform processes this can be a lengthy process where any improvements in time lines impact directly on the time to manufacture. A number of commercially available Micro Bioreactor Systems are available which can facilitate a more holistic approach to cell line evaluation in which cell line screening is combined with an investigation of process parameters.
This talk will describe the potential incorporation of the Micro-24 Bioreactor system (Pall) into cell line evaluation for the production of biopharmaceutical molecules using mammalian or microbial processes. Data obtained in Micro-24 Bioreactors will be compared with that obtained from conventional systems to demonstrate the utility of scale down systems for cell line screening. The relative advantages and limitations of such an approach will be considered. Furthermore the ability of scale down systems to predict cell line performance in stirred tank bioreactors will be discussed.
11:45 – 12:00 Use of micro scale mammalian cell culture in process development
Dr Adrian Haines, Lonza Biologics, UK
12:00 – 12:15 Functionalising cell signalling in microscale production of differentiated cells and tissue engineering
Dr Yuen Ling Ng., University of Nottingham, UK
12: 15– 13:45 Lunch and trade show
This is also a good time to fill out your feedback forms
13:45 – 14:30 Question and Answer Session
Delegates will be asked to submit questions to a panel of experts. Questions can be submitted before the event or on the day
14:30 – 15:00 Characterisation of an automated micro bioreactor with CHO cultures: process control, clone ranking, process optimisation and DoE
Dr Barney Zoro, TAP Biosystems, UK
Implementation of high throughput automated systems is recognised as a valuable approach in bioprocess development, facilitating reduction in project timelines and accelerating delivery of new therapeutic molecules to the clinic. Through consultation with industrial experts, TAP Biosystems identified a clear unmet need for a high throughtput microscale bioreactor. Working with leading biopharmaceutical industry partners to test system prototypes, refine specifications and gather system performance data, TAP combined single use and automation technologies to create a microbioreactor platform approach. Laboratory analysis of physical and cell culture performance indicated suitability for relevant bioprocess applications, including clone screening and process optimisation. Subsequent field testing at a range of biopharmaceutical industry laboratories has lead to broad acceptance of the approach and created a range of data driven examples covering key needs in bioprocess development, such as consistency, predictability and increased experimental throughput.
15:00 – 15:30 Afternoon Tea/Coffee and trade show
15:30– 16:00 Small scale fed batch cultivation of Escherichia coli: carbon limited growth in microtiter plates
Csilla Török, Austrian Center of Industrial Biotechnology, Austria
The production of heterologous proteins in Escherichia coli has become a standard technique widely used in biotechnology and for therapeutic applications. The selection of a strain/vector combination for the production of a defined target protein, and the establishment of suitable cultivation/induction conditions are critical and time consuming steps. To accelerate the process optimization high throughput (HTP) applications with high information content are requested. The BioLector, a mini-bioreactor system (m2p-labs GmbH; Baesweiler; Germany) enables online monitoring of cell density via scattered light and measurement of pH and dissolved oxygen tension (DOT) via fluorescence intensities emitted by optodes implemented in shaken microtiter plates. During this work an enzyme based substrate delivery method – the EnBase® Flo cultivation medium (BioSilta Oy; Oulu; Finland) was applied for 1000 µl scale cultivations of E. coli in the BioLector, to establish a stable protocol for highly reproducible “fedbatch” like cultivations. Thereby the impact of the initial optical density of the inoculum, the inoculum preparation (glycerol stock, pre-culture in different media) and of varying enzyme concentrations on growth behavior was investigated. Based on these results a standard cultivation protocol was defined and a reproducibility study with 18 replicates was performed to ensure the well to well and plate to plate reproducibility. Due to the good reproducibility of the chosen standard protocol high throughput characterization of different recombinant host strains or cultivation conditions is enabled.
16:00 – 16:30 High Throughput Screening & Analytics For Rapid Process Development
Dr James Taylor, Pall Life Sciences, Portsmouth, UK
16:30 – 17:00 Chairman’s summing up
This meeting was organised by Euroscicon (www.euroscicon.com), a team of dedicated professionals working for the continuous improvement of technical knowledge transfer to all scientists. Euroscicon believe that they can make a positive difference to the quality of science by providing cutting edge information on new technological advancements to the scientific community. This is provided via our exceptional services to individual scientists, research institutions and industry.
About the chair
Gary Lye is Professor of Biochemical Engineering within the Advanced Centre for Biochemical Engineering at University College London (UCL). He is Deputy Head of Department and Director of the Industrial Doctoral Training Centre (IDTC) in Bioprocess Engineering Leadership. He received his PhD in Biotechnology from the University of Reading in 1992. Between 1993 and 1996 he was successively a Research Fellow and then Lecturer in Chemical Engineering at Imperial College London and the University of Edinburgh. He joined UCL in 1996. He has broad research interests on the application of microscale and automation techniques to rapid bioprocess design, optimisation and scale-up
About the Speakers
Barney Zoro’s primary focus is delivering high quality bioreactor systems which meet or exceed needs in bioprocess development, including implementation of new technology. Barney’s personal and professional goal is to ensure industry wide acceptance and use of progressive bioprocess technologies, demonstrating technical capability and value to businesses through an evidence-based approach. Barney read Chemical Engineering at Cambridge University and continued his study at University College London (UCL) with a Masters degree and a Doctorate in Biochemical Engineering, with a focus on Tissue Engineering. He has published papers in Biotechnology and Bioengineering, presented various conference posters and talks and has lectured at UCL.
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