Bioprocess miniaturization: Development and optimisation

Tuesday, 26 November 2013

This is a Euroscicon Small Conference,  an outline of the day can be found at

Bioprocess miniaturization: Development and optimisation
Tuesday, 26 November 2013 09:00 - 17:00

Cineworld: The O2
Peninsula Square
SE10 0DX
United Kingdom

Map and Directions
Miniaturisation and automation of bioprocess development continues to be a rapidly expanding area of interest since the technologies promise to reduce biopharmaceutical development time and cost. This meeting will focus on recent technologies used in high throughput bioprocess development, from clone selection through to analysis of final product and formulation. Expert speakers will describe the development and use of current miniaturisation technologies together with the  technical and regulatory hurdles that must be overcome to facilitate wider industrial uptake.

This event has CPD accreditation and is part of  The 2013 BioProcessing Summit -

Meeting chair: Professor. Chris Lowe, Department of Chemical Engineering and Biotechnology, University of Cambridge, UK

Who Should Attend:

Biotech and Pharma Industry: CEOs, Chief Scientists, Group Heads, Senior and Junior Scientists, Research Managers

Academic and Research Institutes: Group and Lab Heads, Postdoctoral Scientists and Research Students

The deadline for abstract submissions for oral and poster presentation has now passed.

Talk times include 5 – 10 minutes for questions

9:00 – 9:45          Registration

9:45 – 10:00        Introduction by the Chair:  Professor. Chris Lowe, Department of Chemical Engineering andBiotechnology, University of Cambridge, UK

10:00 – 10:30      Confinement phenomenon based bio-process intensification using monolithic microreactors
Professor Galip Akay, School of Chemical Engineering and Advanced Materials, Newcastle University, UK
Bio-Process intensification, B-PI  (by a factor of 5-200 fold compared with batch processing) is achievable using monolithic microreactors with well defined  physical and biochemical structure of pores used as support for bacteria or cells. This enhancement is due to 'confinement phenomenon' in which the behaviour of microorganisms is dependent on their confining environment.  Recent examples of B-PI  pioneered at Newcastle University include fermentation, antibiotics and enzyme production as well as tissue engineering and Agri-Process Intensification through bacterial nitrogen fixation by plants. Mechanism of B-PI will be discussed.

10:30 – 11:00      Microfluidic BioLector – A new microbioreactor platform with continuous pH-control and substrate feeding
Dr. Frank Kensy and Dr. Christian Hetzel, m2p-labs GmbH/Inc., Germany/USA
Today bioprocess development is mainly performed in lab-scale stirred tank bioreactors due to controlled processconditions provided by these reactors. Even if several microbioreactor concepts have been established so far in industry to fulfill the demand of high-throughput and ease of use, these technologies are mainly applied in clone screening and media development due to the lack of pH-control and feeding. Therefore, m2p-labs amplified the spectrum of their microbioreactor technology, BioLector®, towards pH-control and continuous feeding options. A user-friendly, disposable microfluidic bioreactor system was created that allows scalable, fully monitored and fully controlled fermentations at micro-scale.

11:00 – 11:30     Speakers’ photo then mid-morning break and poster exhibition and trade show

11:30 – 12:00     Lyophilised Biopharmaceticals- Looking at Cake Properties
Dr Daryl R Williams, Director of Development, Discovery Space and Reader in Particle Science, Department of Chemical Engineering, Imperial College, UK
An innovative method for the mechanical testing of freeze-dried biopharmaceutical cakes in situ vials has been developed. This simple and quick compression test allows a range of cake mechanical properties to be assessed quantitatively. It can be readily applied to fragile and moisture sensitive freeze-dried cakes within the vials. Freeze dried mannitol, sucrose and trehalose samples all yielded linear compressive elastic behavior for small strains with Young's Moduli of 25, 120 and 170 kPa respectively. This method discriminates readily between the three excipients reported here and can be used to optimise formulation of biopharmaceutical systems.

12:00  – 12:30   Up-Scaling From A Micro- To A Lab-Scale Bioreactor By Applying The Fed-Batch Mode At BothScales
Csilla Török, ACIB GmbH – Austrian Centre of Industrial Biotechnology; c/o University of Natural Resources and Life Sciences, A-1190 Vienna, Austria;  Monika Cserjan and Gerald Striedner, Department of Biotechnology, University of Natural Resources and Life Sciences, A-1190 Vienna, Austria.

12:30  – 13:30     Lunch, poster exhibition and trade show

13:30 – 14:30      Discussion session
This discussion session is an informal question and answer session.  This is an ideal opportunity to get advice and opinionfrom experts in this area.  This session is not for questions about specific talks, which can be asked after the speakerssession, but for discussing either general topics or specific issues.There are three ways you can ask questions:
1.    Before the session you can submit your question to Euroscicon staff at the registration desk,
2.    Before and during the session you can submit a question or comments, by email, which will be provided on the day ofthe event
3.    During the session you can put your hand up and join in

14:30 – 15:00      ambr™ and ambr250™: advanced tools for automated optimization and process development inboth microbial and cell cultures for application in biotherapeutics and industrial biotechnology
Mr Mwai Ngibuini, Bioprocess product specialist, TAP Biosystems, UK
Exploration of a large number of cell lines or microbial strain candidates, as well as developing optimal process pathways requires the application DOE analysis with a suitable power number. Traditionally these campaigns require large laboratory space, and are both costly and labour intensive. TAP Biosystems has developed the advanced microbioreactor (ambr™) that is widely adopted as a rapid cell line selection and early stage cell culture optimization tool. As well as, the ambr250™, a tool that is being used for microbial strain selection, process optimization and late stage cell culture optimization. Here we present a technology overview and industry derived data.

15:00 – 15:30      Afternoon Tea, last poster session  and trade show


15:30 – 16:00      Introducing: micro-Matrix, the next generation in microbioreactors
Mr Martijn Kreukniet, Product Manager, Applikon Biotechnology, UK
Applikon biotechnology specializes in development, manufacturing and marketing of bioreactor systems from production scale to laboratory scale. What are the trends and the challenges in the market when scaling down, what are the currently available solutions and where do we see the new developments take us?  What is the current volume limit of the commercial small-scale bioreactors and why. Applikon offers systems down to 200 microliter volume. When scaling down to this volume the main process parameters should be maintained. Oxygen supply, mixing, nutrient supply are just a few parameters but also sample volume for proper analysis is important. Time to setup an experiment is another factor that will determine the success of a small-scale system. When a successful mini or micro bioreactor is developed and operational, the next challenge is to cope with the large amount of process data that will be generated by these systems. How can we make it easier to define multiple experiments and to interpret the experimental data. This presentation will aim to give an overview of the bioreactor market in general with a focus on the scale down market.

16:00 – 16:30     Using an automated microscale processing approach for generation of scaleable design data
Dr Martina Micheletti, Senior Lecturer, Biochemical Engineering UCL, London, UK
Coupling high throughput microscale techniques with the operation of automated laboratory platforms offer valuable opportunities in both upstream and downstream processing. The talk will show how the approach has been successfully applied to different scenarios, namely an industrial pharmaceutical process using Baeyer-Villiger monooxygenases for antibiotic synthesis and the optimisation of a protein refolding step from inclusion bodies. In both cases the microwell-based linked process sequence develop within the robotic platform enabled faster identification and characterisation of optimal conditions, showing good reproducibility over multiple runs and excellent scalability to larger scales of operation.

16:30 - 17:00      Chairman’s summing up

Registration Website:

Keywords:  bioreactor, micro, automated, culture, single use, Microbioreactors, Well Plate, Liquid Feed, bioprocesses, fermentation, cell culture, miniature bioreactor, Antibiotics, Bio-microreactors, Bio-Process Intensification,  Enzymes,  Fermentation, lyophilisation, biopharmaceuticals, Freeze drying, mechanics, formulation, High through put, scalability, automation, single use, bioreactors, small scale, bioreactor, miniaturization,scale down model, parallel bioprocesses, screening, downscaling, micro fermentation, micro bioreactor, microwell, bioprocessing, biocatalysts, refolding, inclusion bodies.

A meeting report from this event will be published in Pharmaceutical Bioprocessing


Contact Details

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