This is a Euroscicon Small Conference, an outline of the day can be found at
This event has CPD accreditation and is part of The 2013 BioProcessing Summit - www.BioprocessingSummit2013.com
Meeting chair: Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, UK
9:30 – 10:15 Registration
10:15 – 10:30 Introduction by the Chair: Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, UK
10:30 – 11:15 Fitting non-platform processes into platform timelines
Dr Alison Tang, Associate Scientist II, MedImmune Ltd, Cambridge, UK
As therapeutic proteins become more complex, the purification challenge increases; in addition, the time allocated for process development reduces due to an ever expanding portfolio. To alleviate the problem, high throughput process development and automation was implemented. In the meeting the challenges and process development tools will be discussed.
11:15 – 12:00 Semi-selective protein precipitation using salt-tolerant copolymers for industrial purification of therapeutic antibodies
Mr Florian Capito, Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Germany
Semi-selective precipitation employing customized salt-tolerant ionic copolymers was evaluated as antibody (mAb) purification step. Precipitated mAb was easily re-dissolved in small volume, enabling concentrating mAb up to hundred-fold, while residual polymer could be removed to > 98 %. mAb recovery of > 90 % and host cell protein clearance of > 80 % were achieved, not requiring any pre-dilution of the cell culture fluid. Precipitation showed no impact on mAb binding affinity. Compared to protein A based purification, yield and purity were lower; yet for high titer processes already being implemented, precipitation is more cost-effective and easier to scale.
12:00 – 13:15 Lunch, poster exhibition and trade show
13:15 – 14:15 Discussion session
This discussion session is an informal question and answer session. This is an ideal opportunity to get advice and opinion from experts in this area. This session is not for questions about specific talks, which can be asked after the speakers session, but for discussing either general topics or specific issues. There are three ways you can ask questions:
1. Before the session you can submit your question to Euroscicon staff at the registration desk,
2. Before and during the session you can submit a question or comments, by email, which will be provided on the day of the event
3. During the session you can put your hand up and join in
14:15 – 14:45 Afternoon Tea, last poster session and trade show
14:45 - 15:30 Liquid-liquid Extraction of Biomolecules; have they a role to play in current bioprocessing strategies?
Dr Jonathan Huddleston, Consultant Biochemical Engineer, Advanced Bioprocessing Centre, Brunel University, UK
Liquid liquid extraction of biomolecules has periodically been promoted as an alternative to packed bed chromatography in downstream processing. The nature and properties of aqueous biphasic extraction systems will be discussed and their potential for application in the downstream recovery of bioproducts will be assessed. Potential applications in biomolecular analysis will also be briefly considered.
15:30– 16:15 Bacillus protein secretion, an aid to downstream processing
Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, UK
Bacillus species are prolific secretor of proteins that are directed into the culture medium. The main secretion pathway, the Sec translocase, requires targeted secretory proteins to be in a translocation competent, substantially unfolded, state. Consequently, secretory proteins must fold as they emerge on the trans side of the membrane. While this has the advantage of facilitating their folding into their native and therefore functional configuration, it adds an element of vulnerability to proteins that intrinsically fold slowly. Approaches to address this issue and improve the yield of proteins in the culture medium will be discussed.
16:15 – 16:30 Chairman’s Summing Up and Close of Meeting
Phone: 07507 799380
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An event from European Scientific Conferences - Euroscicon "Specialising in communicating cutting edge technology & methodology in the Life Sciences"
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Company number: 4326921, Trading Address: Euroscicon Ltd, Highstone House, 165 High Street, Barnet, Herts. EN5 5SU, UK. Registered Office: 47 High Street, Barnet, Herts, EN5 5UW, UK