Recovery and purification of biosynthetic products: Downstream processing for the 21st century

Wednesday, 27 November 2013

This is a Euroscicon Small Conference,  an outline of the day can be found at

Recovery and purification of biosynthetic products: Downstream processing for the 21st century
Wednesday, 27 November 2013 09:00 - 17:00

Cineworld: The O2
Peninsula Square
SE10 0DX
United Kingdom

Map and Directions
The growing interest in biotechnology development and the high demand for biopharmaceuticals have contributed to the growth of the global biopharmaceutical market, which now comprises over 200 products. This market has reached $106 billion in 2009 and is forecasted to grow with a CAGR of 11.2% by 2016. The main challenges of the biopharmaceutical industry now are to increase the efficiency and reduce the costs of the manufacturing process, with respect to the protein recovery, purity and efficacy. Therefore, new strategies are being implemented for the upstream and downstream processing. In the upstream, genetic engineering and glycoengineering approaches, as well as single-use bioreactors are being developed and applied. However, as culture yields are continuously increasing, the bottleneck in the manufacturing process has shifted to the downstream processing, which accounts for ~ 80% of the total production cost. Many aspects are being considered for improving economics in downstream processing, such as simplification of the chromatographic purification processes, application of membrane technology, crystallization and precipitation. This meeting will focus on the advances, innovation and challenges in the area of downstream processing of biopharmaceuticals.

This event has CPD accreditation and is part of The 2013 BioProcessing Summit -

Meeting chair: Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, UK

9:30 – 10:15 Registration

10:15 – 10:30   Introduction by the Chair: Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, UK

10:30 – 11:15 Fitting non-platform processes into platform timelines

Dr Alison Tang, Associate Scientist II, MedImmune Ltd, Cambridge, UK

As therapeutic proteins become more complex, the purification challenge increases;  in addition, the time allocated for process development reduces due to an ever expanding portfolio.  To alleviate the problem, high throughput process development and automation was implemented. In the meeting the challenges and process development tools will be discussed.  

11:15 – 12:00 Semi-selective protein precipitation using salt-tolerant copolymers for industrial purification of therapeutic antibodies

Mr Florian Capito, Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Germany

Semi-selective precipitation employing customized salt-tolerant ionic copolymers was evaluated as antibody (mAb) purification step. Precipitated mAb was easily re-dissolved in small volume, enabling concentrating mAb up to hundred-fold, while residual polymer could be removed to > 98 %. mAb recovery of > 90 % and host cell protein clearance of > 80 % were achieved, not requiring any pre-dilution of the cell culture fluid. Precipitation showed no impact on mAb binding affinity. Compared to protein A based purification, yield and purity were lower; yet for high titer processes already being implemented, precipitation is more cost-effective and easier to scale.

12:00 – 13:15 Lunch, poster exhibition and trade show

13:15 – 14:15 Discussion session

This discussion session is an informal question and answer session.  This is an ideal opportunity to get advice and opinion from experts in this area.  This session is not for questions about specific talks, which can be asked after the speakers session, but for discussing either general topics or specific issues. There are three ways you can ask questions:

1.   Before the session you can submit your question to Euroscicon staff at the registration desk,

2.   Before and during the session you can submit a question or comments, by email, which will be provided on the day of the event

3.   During the session you can put your hand up and join in

14:15 – 14:45    Afternoon Tea, last poster session and trade show

14:45 - 15:30 Liquid-liquid Extraction of Biomolecules; have they a role to play in current bioprocessing strategies?

Dr Jonathan Huddleston, Consultant Biochemical Engineer, Advanced Bioprocessing Centre, Brunel University, UK

Liquid liquid extraction of biomolecules has periodically been promoted as an alternative to packed bed chromatography in downstream processing. The nature and properties of aqueous biphasic extraction systems will be discussed and their potential for application in the downstream recovery of bioproducts will be assessed. Potential applications in biomolecular analysis will also be briefly considered.

15:30– 16:15    Bacillus protein secretion, an aid to downstream processing

Professor Colin R. Harwood, Centre for Bacterial Cell Biology, Institute for Cell & Molecular  Biosciences, Newcastle University, UK

Bacillus species are prolific secretor of proteins that are directed into the culture medium. The main secretion pathway, the Sec translocase, requires targeted secretory proteins to be in a translocation competent, substantially unfolded, state. Consequently, secretory proteins must fold as they emerge on the trans side of the membrane. While this has the advantage of facilitating their folding into their native and therefore functional configuration, it adds an element of vulnerability to proteins that intrinsically fold slowly. Approaches to address this issue and improve the yield of proteins in the culture medium will be discussed.

16:15 – 16:30   Chairman’s Summing Up and Close of Meeting

A meeting report from this event will be published in Pharmaceutical Bioprocessing


Contact Details

Payment Instructions

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    You can pay by - Credit card, Cheque, Purchase Order or Bank transfer.
    Payment must be received prior to the meeting

    Credit card
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    Cheque Payment
    Cheques should be made payable to Euroscicon and mailed (together with a print out of the invoice which will be available at the end of the registration process).  After registration you will be emailed details of where to send your cheque.

    Purchase Order

    Please input your Purchase Order number in the box provided once you have selected your mode of payment and an invoice will be sent to you accordingly

    Bank transfer
    Clients organizing bank transfers from non-UK banks will need to pay an additional administration fee of £15.00. Please ensure that you add this fee onto your payment to ensure successful registration.  
    After registration you will be emailed details of where transfer your money.

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