Identifying T Cell Subset Phenotype and Function: alpha/beta, gamma/delta, regulator, helper, NK & cytotoxic T cells

Hertfordshire
Friday, 16 March 2007

Identifying T Cell Subset Phenotype and Function: alpha/beta, gamma/delta, regulator, helper, NK & cytotoxic T cells
Friday, 16 March 2007 09:00 - 17:00 (GMT)

BioPark Hertfordshire
Broadwater Road
Welwyn Garden City
Hertfordshire
AL7 3AX
United Kingdom

Map and Directions

"The diversity of T cell subsets and functions makes it imperative that they can be identified, characterised, propagated, traced in vivo and their function elucidated effectively. This one day EuroSciCon meeting will provide the ideal opportunity to hear both the commercial companies presenting new techniques and academics highlighting their latest findings in this area. Similarly, there will be opportunities throughout the day for the audience to interact with these speakers and discuss their results in an informal setting."
Chair- Dr Catherine Derry, Science Communicator
 


Abstracts will be accepted for oral or poster presentation and will be published in the meeting proceedings

09:00 – 09:45 Registration - Tea/Coffee

09:15 – 09: 35 *Tour of the BioPark by William Sprigings(Marketing Director) or Steve Read (General Manager)

09:45 – 10:00 Introduction by the Chair: Dr Catherine Derry, Science Communicator

10:00 – 10:30 Identifying T Cell Subset Phenotype and Function: alpha/beta, gamma/delta, regulator, helper, NK& cytotoxic T cells Professor Adrian Hayday, King’s College London, School of Medicine, UK
Throughout vertebrate evolution two sets of T lymphocytes have been conserved, these bearing aß T cell receptor and ?d T cell receptors, respectively. The alpha/beta T cell compartment is known to provide pathogen specific immunity that can be exploited to fight tumours but that can malfunction and cause autoimmune disease. By contrast the gamma delta compartment is less well understood. Data to be presented will consider key involvements of ?d cells in immune responses and their promising application in immunotherapy.

10:30 – 11:00 Magnetic Separation for T cell Subset Sorting & Identification Dr Dawn Farrar, Miltenyi Biotec, UK
Miltenyi Biotec’s MACS® cell separation systems are widely regarded as the “gold standard” in magnetic cell separation. In this talk I will give a brief overview of the many different options we have available to sort a wide variety of T cell subsets. In addition to our widely used cell separation reagents Miltenyi Biotec also now offer a range of products to allow activation, expansion and culturing of T cells down stream which will also be discussed.

11:00 – 11:30
Morning Tea/Coffee and poster viewing

11:30 – 12:00 Tracking the interplay between antigen-specific naïve and regulatory T cells in vivo
Dr Jian-Guo Chai, Faculty of Medicine, Imperial College of Science, Technology & Medicine, UK
There is intensive debate about whether CD4 25 Foxp3 Treg cells can be induced in vivo from non-regulatory, naive CD4 T cells. Furthermore, little is known about the in vivo behaviour of self-antigen Treg cells in periphery. Moreover, current knowledge of the cellular and molecular parameters of regulation of the interplay between self-antigen specific-naive and -Foxp3 Treg cells in vivo is also very limited. To address these issues, we have designed an in vivo model, taking the advantage of the availability of H2Ab-restricted, HY-specific TCR transgenic mice as well as syngeneic Rag-deficient B6 mice. The key findings will be presented and their significance will be discussed.

12:00 – 12:30 Adoptive cell therapy with “customized” antigen-specific CD4 CD25 Tregs
Dr Giovanna Lombardi, King’s College School of Medicine, UK
Naturally arising CD4 CD25 regulatory T cells play a pivotal role in the prevention of autoimmunity. The phenotype and function of Tregs is under investigation. New markers that allowed the identification of subtypes of Tregs have been recently discovered. The function in vivo of these different subtypes of Tregs is under investigation. Tregs with alloantigen-specifiicity as potential reagents for adoptive cell therapy in promoting donor-specific transplantation tolerance have been generated and expanded in vitro by either using DCs pulsed with the relevant alloantigen or TCR transduction. Their suppressive potential has been compared to polyclonal Tregs both in vitro and in vivo. The results obtained so far suggest that they regulate and are more effective than polyclonal Tregs.

12:30 – 13:00 Functional characterization of CD4 CD25 regulatory T cells - fundamental mechanisms and implications for the pathogenesis of autoimmune
disease Dr Oliver Garden, Imperial College, London
The protean functions of CD4 CD25 FoxP3 regulatory T cells (Tregs) have earned them a place on the centre-stage of contemporary immunology. We have developed a quantitative assay of Treg function in vitro that has disclosed defective interactions of conventional and regulatory T cells in mouse models of systemic lupus erythematosus and inflammatory bowel disease. We have also developed a method for labelling murine T cells with ultrasmall iron oxide nanoparticles that preserves proliferative, migratory and regulatory functions in vitro, offering the potential in the future to allow longitudinal studies of lymphocyte migration in vivo by ultra-high resolution magnetic resonance imaging.

13:00 – 14:00
Lunch and poster viewing

14:00 – 14:30 A TRAP assay (Trogocytosis Analysis Protocol) to detect, quantify, characterize and purify antigen specific live lymphocytes by flow cytometry, via their capture of membrane fragments from antigen presenting cells Denis Hudrisier, IPBS, France
We describe an approach allowing the detection, quantification, separation and characterization of T and B cells reactive to specific antigens on a functional basis by flow cytometry. The assay is based on the property of lymphocytes to capture membrane components from the cells they interact with, in a process we call trogocytosis. Our method allows the simultaneous quantitative identification of reactive CD8 , CD4 and B cells. In addition it offers a simple and general alternative to other methods previously described to detect and quantify lymphocyte reactivity, and it can also be used in combination with those.

14:30 – 15:00
Immune Response in Pregnancy: an inflammatory view Professor Ian Sargent, University of Oxford, UK
For many years reproductive immunology has been dominated by the “Th1/Th2” hypothesis in which the fetus avoids maternal T cell rejection through a bias towards Th2 cytokine production. The discovery that normal pregnancy is a controlled state of inflammation, both early on at the implantation site and later systemically, has challenged this concept, as has the finding that the predominant immune interactions in the decidua are between placental trophoblast and maternal NK cells rather than T cells. We suggest novel ways in which trophoblast/NK cell interaction could stimulate the systemic inflammatory response and lead to the maternal syndrome of pre-eclampsia.

15:00 – 15:30
Afternoon Tea/Coffee and last poster viewing

15:30 – 16:00 Chronic Trichuris muris infection suppresses skin allergen responses and Langerhans cell migration  Mr Neil Humphreys, University of Manchester, UK
Chronic infection of the intestinal dwelling nematode Trichuris muris is associated with T cell Th1 polarisation and a mild caecal pathology which is regulated via IL-10. The chemical allergen 2,4-dinitrochlorobenzene (DNCB) elicits a Th1 response whereas Trimellitic anhydride (TMA) drives Th2 polarisation. Using this skin allergy model we demonstrate T. muris infection systemically dampens the DNCB induced allergy, whereas the response to TMA was unaltered. Suggesting T. muris induced regulation preferentially regulates remote Th1 responses. Currently the role epidermal Langerhans cell migration plays in elicitation and regulation of the allergic response in the context of parasite infection is being investigated.

16:00 – 16:30 Instruction of effector CD4 T lymphocytes by dendritic cells; the power of flow cytometry in immunological research, Dr Joost Schuitemaker, IQ Products
Since the introduction of flow cytometry in the seventies many new applications have been developed to use in different fields of interest. Most of these applications are based on antibodies and all are characterized by the use of fluorescent dyes. During this presentation several techniques are presented that are used in fundamental immunological research. The work of several academic groups is presented that try to unravel the mystery of the development of the different circulating CD4 positive T cell subsets. By making use of in vitro culture models and flow cytometry the different subsets can be characterized, even in complex mixtures of cells.

16:30  Chairman’s summing up & close.

17:00 – 17: 20 *2nd Tour of the BioPark

 

*Please note that a tour for exhibitors is scheduled for 2:15- 2:45

Fees

  • Standard - £485.00
  • Academic - £298
  • Student - £198

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