Discussion Workshop: Improving immunohistochemistry 2010

Welwyn Garden City, Hertfordshire
Friday, 08 October 2010

An event from European Scientific Conferences - Euroscicon "Specialising in communicating cutting edge technology & methodology in the Life Sciences"  Linked In

Discussion Workshop: Improving immunohistochemistry 2010
Friday, 08 October 2010 09:00 - 17:00

BioPark Hertfordshire
Broadwater Road
Welwyn Garden City
Hertfordshire
AL7 3AX
United Kingdom

Map and Directions

 This event is a discussion workshop.  We have invited 6 experts to discuss their work in an informal lecture setting, discussion and demonstration groups, one2one sessions and panel discussions

 

Meeting Chair: Dr Will Howat, Cambridge Research Institute, Cancer Research UK

 

This event has CPD accreditation

On registration please submit your questions to the panel that will be asked by the chair on the day of the event

 

9:00 – 9:30          Registrationp

 

9:30 – 9:45          Introduction by the Chair: Dr Will Howat, Cambridge Research Institute, Cancer Research UK

 

Talks by Invited Experts:

 

9:45 – 10:00        Key Steps in Antibody Validation

Dr Hayley Whitaker

Cambridge Research Institute, Cambridge, UK

 

10:00 – 10:15      High Throughput IHC Antibody Validation

Mr Richard Bystry

Asterand, UK

 

10:15 – 10:30      IHC trouble shooting

Dr Chris van der Loos

Academic Medical Center, Amsterdam, The Netherlands

 

10:30 – 10:45      ISH troubleshooting

Dr Howard Pringle

University of Leicester, UK

 

10:45 – 11:00      Tissue cross reactivity

Miss Jo Mitchell

Huntingdon Life Sciences, UK

 

11:00 – 11:15      Approach to image analysis solutions

Mrs Alison Bigley

AstraZeneca, UK

 

11:15 – 11:20      Participant Photo

 

11:20 – 11:45      Mid-morning Break

 

11:45 – 12:45     Question and Answer Session

 

12:45  –              Working Lunch (in Exhibition Area)

 

13:00 – 16:30     Discussion Groups and One to One Sessions

 

Round table discussion groups will be held in the exhibition area throughout the afternoon

One to one sessions will be held in a break-out room after lunch (in parallel to the discussion groups)

Where appropriate delegates will be able to bring their samples to the discussions   

 

Table 1: Image Capture Instumentation

Hosted by Will Howat, who graduated with a BSC (Hons) in Immunology & Pharmacology from the University of Strathclyde, before gaining a PhD in Pathology from the University of Southampton. After two post-doctoral positions in Southampton, he moved to the Wellcome Trust Sanger Institute in Cambridge as the leader of Research & Development for the Immunohistochemistry group of the Atlas of Protein Expression project. He is now with Cancer Research UK as the head the Histopathology/ISH facility at the Cambridge Research Insititute

 

Table 2: Antibody Validation

Hosted by Hayley Whitaker, who completed a B.Sc. in Biochemistry in Manchester before completing a BBSRC funded Masters by research in Biotechnology in Canterbury. After a short period working at the Sanger Centre on the Human Genome Project she began her PhD in prostate cancer at Imperial College. Hayley has worked on prostate cancer in Cambridge since 2004, focusing on rapid biomarkers validation and development. She is expanding the biomarker pipeline at the Cancer Research UK Cambridge Research Institute into include pancreatic, ovarian and lung cancers as well as continuing to work on prostate cancer.

 

Table 3: IHC Troubleshooting

Hosted by Chris van der Loos, who trained as histotech and prepared a PhD thesis in 1992 as first in the AMC. Major interest in the technical issues of IHC: fixation, tissue handling, IHC detection techniques, multiple staining and spectral imaging.  

 

Table 4: ISH Troubleshooting

Hosted by Howard Pringle, who graduated from Bristol (1979) in Applied Biological Sciences and completed a PhD in Biotechnology at the University of Warwick (1983). He studied DNA replication as a post-doctoral scientist in the Department of Molecular Biology, University of Edinburgh. Then moved to University of Leicester in 1985 as Non-clinical Lecturer in the Department of Pathology. He is currently a Reader in Molecular Pathology in the Department of Cancer Studies and Molecular Medicine at Leicester. His research includes molecular studies of skin, breast and colorectal cancer.  He has developed and applied non-isotopic in situ hybridisation methods on human and animal tissues since moving to Leicester in 1985.

 

Table 5: Tissue Cross Reactivity

Hosted by Jo Mitchell, who holds a BSc. honours in Applied Biology with European Studies and a MSc. in Biomedical Science. She has over 12 years experience in IHC, 5 years gained at Pharmagene working on pre-clinical research projects, 3 years spent managing the day-to-day operations of 'The Atlas Project' high-throughput IHC lab at The Sanger Institute and 2 years spent helping to set-up and run the CR-UK Histopathology/IHC Core Facility at the CRI. Jo joined HLS in 2008 as Head of Special Pathology Services (Safety Assessment: Pathology Division). She assumes scientific responsibility for IHC (including tissue cross-reactivity), electron microscopy and micro-autoradiography studies

 

Table 6: Image Processing

Hosted by Alison Bigley, a Fellow and Chartered Scientist of the Institute of Biomedical Science. She has 25 years experience in histology, IHC and electron microscopy and 20 years experience in image analysis. She began her career in Histopathology at hospitals in the West Midlands and Cardiff and is currently the Head of Analytical Morphology (Pathology Dept) in Safety Assessment at AstraZeneca Pharmaceuticals. Her current area of research is mainly within oncology but also leads method development and problem solving studies for diabesity and vascular modulation projects. Alison also lectures at Birmingham University on Image analysis in Drug Discovery for the Physical Sciences of Imaging in the Biomedical Sciences doctoral training.

 

Table 7: High throughput IHC antibody validation

Hosted by Richard Bystry, who since graduating in 1994 has been working with immunohistochemistry and in situ hybridisation. Working at the University of Hertfordshire he first encountered immunofluorescence. At The Lab of Molecular Biology in Cambridge he used ISH and IHC to aid in the investigation of the role of regulatory T cells in the immune response. Richard works at Asterands UK site in Royston, Hertfordshire where he is a Senior Scientist in the Molecular Pathology group working as a CRO supporting drug discovery efforts for many of the worlds largest Pharmaceutical companies still using IHC and ISH.

 

16:30 – 17:00      Expert Panel's Summing Up

 

17:00                   Chairman’s Summing Up

 

 

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This meeting was organised by Euroscicon (www.euroscicon.com), a team of dedicated professionals working for the continuous improvement of technical knowledge transfer to all scientists. Euroscicon believe that they can make a positive difference to the quality of science by providing cutting edge information on new technological advancements to the scientific community. This is provided via our exceptional services to individual scientists, research institutions and industry.

 
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    Euroscicon
    BioPark Hertfordshire
    Broadwater Road
    Welwyn Garden City,
    Hertfordshire AL7 3AX
    United Kingdom


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