'Assigning gene function - novel technologies and high throughput'

Friday, 16 June 2006

'Assigning gene function - novel technologies and high throughput'
Friday, 16 June 2006 09:30 - 16:30 (GMT)

Birkbeck College
Basement Lecture Theatre
43 Gordon Square
United Kingdom

Map and Directions

This will be an exciting meeting which will cover novel genomic technologies and the use of bioinformatics in high throughput data analysis. It will feature academic and industry seminars.
Meetings Chair: Dr. Irina Udalova, Kennedy Institute of Rheumatology, London U.K.

9.30 -10:00  Registration

10:00 – 10:15  Introduction by the Chair:   Dr. Irina Udalova, Kennedy Institute of Rheumatology, London U.K.

10:15 - 10: 45 Genome-wide genetic association of multiple complex phenotypes in outbred mice  Dr Richard Mott - Wellcome Trust Centre for Human Genetics, University of Oxford
The genetic basis of quantitative phenotypic variation in mice has proved difficult to dissect at a molecular level, largely frustrated by the difficulties of fine-mapping quantitative trait loci (QTLs). Here we report genome-wide high resolution mapping in over 2,000 genetically heterogeneous stock mice of 843 QTLs, identified with an estimated specificity of 71% and sensitivity of 89% and were resolved into an average 95% confidence interval of 2.8 megabases.   The QTLs contribute to variation in three models of human disease (asthma, type 2 diabetes, anxiety) as well as measures of immunology, biochemistry and haematology.

10:45 - 11:15  Proteomic strategies; a tool for gene function assignment  Dr Robin Wait, Imperial College London
Proteomic approaches can reveal the key components of complex and unknown biological systems, without being limited by prior hypotheses, or requiring specific detection reagents such as antibodies or oligonucleotide probes.
Although primarily a tool for protein expression analysis, in contrast to transcriptomic approaches protein modifications, interaction partners and subcellular locations can be determined, which are not deducible a priori from genome sequences, and may provide clues to protein function. Proteomic strategies are thus a valuable adjunct to complementary bioinformatic, immunochemical and nucleic acid based approaches.

11:15 – 11:45  Mid-morning break

11:45 – 12:15 Transcriptome changes within the hypothalamo-neurohypophyseal systemin response to dehydration  Mr Charlie CT Hindmarch, Henry Wellcome Laboratories Integrated Neuroscience
We have used microarrays to comprehensively catalog the genes expressed within discrete regions of the HNS in both control and dehydrated rats. Comparison of these gene lists has enabled us to identify transcripts that are regulated as a consequence of dehydration – as well as RNA’s that are enriched in specific regions.

12:15 – 12:45   Enhanced speed and reproducibility of slide based applications using a-Hyb™ Hybridization Station. A fully automated system with active fluidics , Dr Simon Mauch, Miltenyi Biotec, Ltd

12:45 - 13:45    Lunch in the exhibition hall

13:45 - 14:15     Printing protein arrays from DNA arrays  Dr. Farid Khan, Babraham Institute, UK
We have developed a novel system which enables the production of protein arrays directly from immobilised DNA arrays. In this method, cell-free protein synthesis is performed between two solid surfaces; one of which is arrayed with DNA molecules while the other surface carries a specific reagent to capture the translated proteins. Individual proteins are synthesised in parallel from the arrayed DNA and subsequently immobilised through interaction with the protein-capturing reagent on the opposite surface to form a protein array. This technology permits the generation of ‘pure’ protein arrays on demand in a single reaction. It also allows production of multiple copies of a protein array through the repeated use of a single DNA array template. 

14:15 – 14:35         Exploring protein-DNA binding specificities using microarrays  Dr Ioannis Ragoussis - Oxford University
Developing Protein-DNA binding assays as tools for understanding how transcription factors recognize particular DNA sequences is important in order to understand the regulation of gene expression. In recent years microarray technologies have been used in order explore transcription factor specificities at a large scale. In this talk methods for constructing such arrays, to which transcription factors can bind will be presented. Data from interrogating 64,000 sequences to determine affinity of transcription factors will be presented and discussed. Further validation tools such as Surface Plasmon Resonance based assays will be also presented.

14:45 – 15:15        Optimisation of DNA Microarrays  Mr Ben Jackson - Bio-Rad Lab Ltd
Carefully designed 27-mer siRNA can bring about even more potent gene silencing that the corresponding 21-mer species.

15:15 – 15:45           tba

15:45 - 16:15          Close     

Registration fees

Standard fee - £320
Academic fee - £160
Student fee- £80
IBMS members fee - £160

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