Recent Advances in Live Cell Imaging

London
Friday, 03 November 2006

Recent Advances in Live Cell Imaging
Friday, 03 November 2006 09:00 - 17:00

The MI Centre
81–103 Euston Street,
London
NW1 2EZ.

Map and Directions

 "This meeting offers a forum for discussion in the area of Live Cell Imaging and its impact as a major experimental approach in many fields of biology. Recent dramatic advances in imaging technology and labeling techniques have enabled researchers to carry out these tasks with high spatiotemporal resolution using confocal laser scanning microscopes, green fluorescent protein and its derivatives, and image analysis systems. Investigations include dynamic processes of intracellular signaling molecules, cellular structure, protein-protein interactions, protein conformational changes, and the behavior of molecules to their natural environment within the intact cell. The technology provides basic and valuable information for clinical studies, design of therapeutic strategies, advancing and complementing biochemical and structural biology studies. The introduction of single-cell quantitative time-lapse imaging and photobleach techniques will be considered in addition to those employed and improved over the last few years".
Chair - Dr Stephen Rawlings - Cambridge, UK

09:15 – 09:45 Registration – Tea, coffee and biscuits

09:45 – 10:00 Introduction by the Chair: Dr Stephen Rawlings, Cambridge, UK

10:00 – 10:30 Live cell confocal imaging: a view from the trenches, Dr Tim Self - University of Nottingham, UK
This talk will consist of a brief description of confocal microscopy and fluorescence. I will then describe the conditions required to image healthy living cells and finish with some of the live cell projects I am involved in at the ICS.

10:30 – 11:00 Coral Hue: a new era in fluorescent proteins, Dr Jaquie Finn - Stratech, UK
This talk outlines the salient features and applications for the CoralHue family of fluorescent proteins. These proteins are cloned from stony coral and have some significant benefits over the traditional FP’s. Come and listen to the talk to hear of the many different imaging applications that can exploit the properties of these fascinating proteins.

11:00 – 11:30 Morning tea/coffee

11:30 – 12:00 Talk title to be confirmed, Olympus UK Ltd

12:00 – 12:30 High Resolution Structured Illumination Microscopy, Dr Rainer Heintzmann - Kings College London, UK
In structured illumination the sample is illuminated with a number of different patterns of light. In our case this is a series of sinusoidal grids at different grid positions and orientations. Experimental datasets acquired under these conditions and reconstructed results from these data, demonstrating a resolution improvement of up to a factor of two over standard widefield microscopy are presented. The non-linear approach of saturating optical transitions (for structured illumination as well as beam-scanning approaches) has a great potential especially in combination with photo-switchable dyes such as the recently released DRONPA protein by Atsushi Miyawaki’s group.

12:30 – 13:00 Talk title to be confirmed, Dr Treanor – Imperial College, London, UK

13:00 – 14:00 Lunch

14:00 – 14:30 Monitoring ion concentrations using the laser-scanning confocal microscope, Dr Paul Thomas - University of East Anglia, UK
Emphasis will be placed on the practical aspects of imaging ions and small molecules in live cells. In particular, I will present some lessons learnt from monitoring cytosolic calcium in an intact tissue (the human lens), and from the measurement of pH in lysosomal/endosomal compartments in macrophages.

14:30 - 15:00 Advanced microscopy solutions for monitoring the kinetics and impact of drug-DNA targeting in living cells, Dr Errington - University of Cardiff
Quantification and exploration of drug targeting dynamics can be highly informative in the rational development of new therapies and in the drug discovery pipeline. The problems faced include the potential infrequency and transient nature of critical events, the influence of micro-pharmacokinetics on the drug-target equilibria, the dependence on preserving cell function to demonstrate dynamic processes in situ, the need to map events in functional cells and the confounding effects of cell-to-cell heterogeneity. This talk addresses key design concepts for the development of imaging tools used to uncover the complexity of drug-targeting in single cells

15: 00 – 15:30 Afternoon Break

15:30 – 16:00 Using bleaching techniques to study protein dynamics in living cells, Dr Gareth Howell - Faculty Biological Sciences, Leeds University, UK
This will be a brief overview into cell bleaching techniques employed in microscopy and a discussion of how these techniques have been used to study protein dynamics within live cells

16:00 – 16:30 Endoplasmic reticulum, Golgi body and cytoskeletal dynamics in the plant cell,
Dr John Runions - Oxford Brookes University, UK
Components of the plant secretory pathway are highly dynamic. Proteins shuttle from the ER through the Golgi bodies to their cellular destinations and back. A good deal of effort is going into the search for the mechanisms of organellar dynamics. We mark various sub-cellular components with fluorescent proteins to study interactions. I will focus on interactions between the ER, Golgi bodies and the actin cytoskeleton. Motion tracking software allows us to collect enough data on Golgi body dynamics that we can work towards a statistical description of how they move relative to the ER.

16:30 – 17:00 Discussion & Close


 

·       Standard fee - £480
·       Academic fee - £240
·       Student fee - £140
·       IBMS members fee - £240

Abstract submission
The Deadline for abstract submissions is September 10th 2006
Abstract guidelines can be found at
http://www.euroscicon.com/absguidlines.html

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Contact Details

Payment Instructions

    • Payment can be made by Visa or Mastercard online by secure server or by post
    • Cheques should be made payable to Euroscicon and mailed (together with a print out of the invoice which will be available at the end of the registration process) to

    Sally Wheatland
    Euroscicon
    PO Box 49717
    London
    N20 8WH.

    Payment must be received prior to the meeting

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