New Applications for Flow Cytometry

London
Friday, 10 November 2006

New Applications for Flow Cytometry
Friday, 10 November 2006 09:00 - 17:00

The MI Centre
81–103 Euston Street,
London
NW1 2EZ
United Kingdom

Map and Directions

9:15 – 9:45 Registration

9:45 – 10:00 Introduction by the Chair: Mr Ian Dimmick, Institute of Human Genetics University of Newcastle Upon Tyne, UK

10:00 - 10:30   The use of a multiplexed bead array system in a study on graft-versus-host Disease, Dr Xiao-nong WANG – Newcastle University, UK
Brief background of our study in GVHD; Why do we choose to use the multiplex system; Our experience in using the system and some results generated by using the system

10:30 – 11:00  Dissecting the cell cycle: linking flow and imaging applications, Prof Paul J Smith, Department of Pathology, School of Medicine, Cardiff University
The cell can integrate internal and environmental signals into molecular responses that become the recognisable descriptors of a combination of pathways. Not surprisingly, the live eukaryotic cell is becoming an increasing point of focus for drug discovery efforts and for research applications using reporter probes to track dynamic changes in discrete pathways. The presentation will overview the evolving technology for image analysis with flow cytometry capacity. There will be a focus on applications of new eGFP stealth reporters and quantum dot technologies with specific reference to understanding the action of anticancer drugs on the cell cycle.

11:00 – 11:30 Morning Break

11:30 – 12:00  New Applications for Flow Cytometry, Dr Lyle Armstrong, Centre For Stem Cell Biology & developmental Genetics, University of Newcastle Upon Tyne, UK
Human embryonic stem cells have enormous potential therapeutic benefits since they can in theory differentiate into any of the cell types found in the adult body. However, in order to make cell replacement therapy feasible several problems must be overcome most notably the fact that ES cells often differentiate into many types of cells from which the cells of interest must be purified before transplantation can be considered. Flow cytometry is one of the best techniques currently at our disposal to achieve this goal.

 The convenient way of cellular analysis, Thorsten Peters-Regehr, Miltenyi Biotec GmbH
The MACSQuant™ Analyzer a new, compact bench-top flow cytometer for highly sensitive multicolor cell analysis. Three lasers, combined with powerful software, makes for a fast and simple analysis of cells. Also, being equipped with two scatter (FSC, SSC) and seven fluorescence channels, the MACSQuant™ Analyzer is the ideal instrument for MACS® Control applications (optimized evaluation of MACS® Separations), as well as other immunofluorescent analyses. The system includes a MACS® Cell Enrichment Unit that is directly controlled by the MACSQuant™ analysis software to enable the fully automated processing of samples and cell analysis – crucial in the reliable detection of rare cells. Automation can further be extended to multi-sample processing when combined with the MACS® Sampler for convenient, hands-free operation.

12:30 – 13:00  Multiparametric flow cytometry, Mr.  Ian Dimmick,Institute of Human Genetics, Newcastle
Instrumentation has increased in it’s speed and capability , noteably the sensitivity levels at which we can work and the speed of analysis and sorting . I would like to start with the importance of good quality control and maintainance of flow cytometers , the importance of good colour compensation and the benefits of the detection of multiple parameters from cells situated in the same test tube .

13:00 – 14:00 Lunch


14-00 - 15:00 Multiparametric flow cytometry Mr Ian Dimmick, Institute of Human Genetics University of Newcastle Upon Tyne, UK

15:00- 15:30  A Multi-Laser Approach to Apoptosis, Gary Warnes, Flow Cytometry & Imaging Manager, Institute of Cell & Molecular Science, Queen Mary’s College, London University
The advent of commercial multi-laser flow cytometers allows the combination of numerous flow cytometric apoptosis assays in a multi-functional manner. Multi-laser instrumentation not only permits the detection of phosphotidylserine flipping by annexin V and cell viability but can simultaneously measure superoxide generation and mitochondrial membrane potential changes occurring during apoptosis in a time-dependent manner. The simultaneous detection of numerous apoptotic events by use of multi-laser flow cytometers, allows the investigator to elucidate the order of events involved in apoptosis in a time-dependent manner.

15:30 – 16:00 Applications of novel developments, Norman Maidment, Life Science Discovery & Laboratory Automation, Beckman Coulter UK Ltd

16:00 – 16:30 Guava Technologies: A Revolution in Flow Cytometry – New Advances, Dr Paul Wheeler - Guava Technologies Inc, UK

Guava Technologies Inc is an innovative flow cytometry company recently established and based in the US. Our key aim is to provide Easy to Use Benchtop Flow Cytometry Systems with turnkey solutions. Our key specialisms include: automated cell count and viability, true volumetric absolute cell counting, apoptosis, cell cycle, clinical CD4 monitoring, and most general flow cytometry applications.

16:30 - 17:00 17:00  Discussion & Afternoon Tea

 

Payment Instructions

    • Payment can be made by Visa or Mastercard online by secure server or by post
    • Cheques should be made payable to Euroscicon and mailed (together with a print out of the invoice which will be available at the end of the registration process) to

    Sally Wheatland
    Euroscicon
    PO Box 49717
    London
    N20 8WH.

    Payment must be received prior to the meeting

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